FoxO1 (phospho Ser256) rabbit pAb Antibody

SKU: orb768175

Description

Rabbit polyclonal antibody to FoxO1 (phospho-Ser256)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Drosophila, Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272
• Molecular Weight: 82
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-FOXO1 antibody, anti-FKHR antibody, anti-FOXO1A antibody, anti-Forkhead box protein O1 antibody, anti-Forkhead box protein O1A antibody, anti-Forkhead in rhabdomyosarcoma antibody

Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 Monoclonal Antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FKHR (Phospho-Ser256) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.