FOXN3 Antibody (C-term)

• Catalog Number: orb1932469
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB40992
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 53835 Da
• Target: This FOXN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human FOXN3.
• UniProt ID: O00409
• NCBI: NP_005188.2, NP_001078940.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Forkhead box protein N3, Checkpoint suppressor 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent image of Hela cell stained with FOXN3 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXN3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).FOXN3 immunoreactivity is localized to Nucleus significantly.

All lanes: Anti-FOXN3 Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.