FOXL2 Antibody / Forkhead box protein L2

SKU: orb1823592

Description

FOXL2 is a critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. It prevents trans-differentiation of ovary to testis through transcriptional repression of the sertoli cell-promoting gene SOX9. FOXL2 is mainly expressed in ovary, parathyroid gland and sex cord-stromal tumors. FOXL2 is generally used for diagnosis of sex cord-stromal tumors with Inhibin, Calretinin and SF-1.

Images & Validation

• Tested Applications: ELISA, FACS
• Dilution Range: ELISA (Order BSA-free format for coating),Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the FOXL2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c
• Clone No.: PCRP-FOXL2-1B4
• Immunogen: The protein domain of FOXL2 protein was used as the immunogen for the FOXL2 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of PFA-fixed human HeLa cells with FOXL2 antibody (clone PCRP-FOXL2-1B4) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free FOXL2 antibody (clone PCRP-FOXL2-1B4) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using FOXL2 antibody (clone PCRP-FOXL2-1B4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Quick Database Links

UniProt

P58012