FOXL2 Antibody

• Catalog Number: orb1248891
• Category: Antibodies
• Description: FOXL2 Antibody
• Target: FOXL2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DSKTGALHSRLDL
• UniProt ID: P58012
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO,
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_075555.1

orb1248891 (2 μg/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1248891 (2 ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1248891 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.