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Catalog Number | orb1248891 |
---|---|
Category | Antibodies |
Description | FOXL2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Rat |
Reactivity | Human, Mouse |
Immunogen | The immunogen for this antibody is: C-DSKTGALHSRLDL |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FOXL2 |
UniProt ID | P58012 |
NCBI | NP_075555.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248891 (2 μg/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 μg protein in RIPA buffer). Detected by chemiluminescence.
orb1248891 (2 ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
orb1248891 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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