FOXK2 Rabbit pAb Discontinued

SKU: orb539520

Description

Rabbit polyclonal antibody to FOXK2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 1:500-1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-430 of human FOXK2 (NP_004505.2).
• Molecular Weight: 80kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXK2, ILF, ILF-1, ILF1

Western blot analysis of extracts of Jurkat cells, using FOXK2 antibody (orb539520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts of Mouse brain, using FOXK2 antibody (orb539520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Quick Database Links

UniProt

Q01167

Shanhong Shi,Weiyuan Lin,Jiequn Zhang,Yanling Zheng Emodin reduces the injury of glomerular mesangial cells in lupus nephritis by targeting forkhead protein K2 through miR-96-5p Chinese Clinical Pharmacology and Therapeutics, 28(12), 1331-1338 (2023)

PubMed:

Applications

WB

Reactivity

Mouse