FOXC1 Antibody

• Catalog Number: orb1248947
• Category: Antibodies
• Description: FOXC1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: RTSGAFVYDCSKF
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXC1
• UniProt ID: Q12948
• NCBI: NP_001444.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IR
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248947 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.

orb1248947 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1248947 (3.75 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1248947 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).