Folic acid

SKU: orb1310979

Description

Folic acid

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 59-30-3
• MW: 441.40
• Purity: 99.27%
• Formula: C₁₉H₁₉N₇O₆
• SMILES: NC1=NC(O)=C2N=C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)C=NC2=N1
• Target: Endogenous Metabolite,DNA/RNA Synthesis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.5 mg/mL (5.66 mM);DMSO:25 mg/mL (56.64 mM)

Bioactivity

• Target IC50:
  FRβ:0.0015 µg/mL|FRα:0.0015 µg/mL
• In Vivo:
  METHODS: To investigat the role of Folic acid (1-100 mg/kg) in a behavioral model of depression, Swiss mice were administered Folic acid (1-100 mg/kg) in a Single gavage dose and subjected to the forced swimming test (FST) and tail suspension test (TST). Results: Oral administration of Folic acid reduced immobilization time in the FST (50-100 mg/kg) and TST (10-50 mg/kg). Folic acid produces antidepressant-like effects in the FST and TST.
• In Vitro:
  METHODS: HUVEC cells were treated with Folic acid (2.5-100 µM) for 48 h. Cell proliferation was detected using xCELLigence RTCA real-time cell analyzer. Results: Folic acid increased cell proliferation in the HUVEC cell line using a cytometric index to determin the EC50 dose of 50 µL METHODS: Neural tube explants were treated with Folic acid (90 µM) for 3 h and morphology was observed using microscopy. Results: in the presence of Folic acid, cell migration fro the neural tube explants was detected in 80% o the cultures within 2-3 h the first cell to leav the initial explant wa the first cell to leav the neural tube explant the first cells leavin the initial explants were tightly organized neuroepithelial cells an the explants were firmly attached to the fibronectin layer.
• Cell Research:
  To determin the effect of FA supplementation on BRCA1 and BRCA2 mRNA expression, All cell lines were treated with 0, 25, 50, 75, or 100 nMol/L FA for 72 hours before harvesting in TRI Reagent according to the manufacturer’s instructions. (Only for Reference)

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, EndogenousMetabolite, Folic acid, folic deficiency, Folacin, Folate, Inhibitor, inhibit, neural tube defects, nutrient, ntidepressant-like, DNA Synthesis, DNA/RNA Synthesis, DNASynthesis, Vitamin M, Vitamin B9, Vitamin B-9, Vitamin B 9, VB9, Pteroylglutamic acid, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

135444779