Folic acid

SKU: orb1310979

Description

Folic acid is an essential nutrient crucial for DNA/RNA synthesis and cellular proliferation. It is widely used in research on folate deficiency, including studies of anemia, neural tube defects, and as a tool in modeling acute kidney injury in both in vitro and in vivo experimental systems.

Research Area

Cell Biology, Metabolism Research, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 59-30-3
• MW: 441.4
• Purity: 97.00% (May vary between batches)
• Formula: C₁₉H₁₉N₇O₆
• SMILES: O=C1C=2C(NC=C(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)N2)=NC(N)=N1
• Target: DNA/RNA Synthesis,Endogenous Metabolite
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.5 mg/mL (5.66 mM);DMSO:25 mg/mL (56.64 mM)

Bioactivity

• Target IC50:
  FRβ:0.0015 µg/mL|FRα:0.0015 µg/mL
• In Vivo:
  METHODS: To investigate the role of Folic acid (1-100 mg/kg) in a behavioral model of depression, Swiss mice were administered Folic acid (1-100 mg/kg) in a single gavage dose and subjected to the forced swimming test (FST) and tail suspension test (TST). RESULTS: Oral administration of Folic acid reduced immobilization time in the FST (50-100 mg/kg) and TST (10-50 mg/kg). Folic acid produces antidepressant-like effects in the FST and TST.
• In Vitro:
  METHODS: HUVEC cells were treated with Folic acid (2.5-100 µM) for 48 h. Cell proliferation was detected using xCELLigence RTCA real-time cell analyzer. RESULTS: Folic acid increased cell proliferation in the HUVEC cell line using a cytometric index to determine the EC50 dose of 50 µL. METHODS: Neural tube explants were treated with Folic acid (90 µM) for 3 h and morphology was observed using microscopy. RESULTS: In the presence of Folic acid, cell migration from the neural tube explants was detected in 80% of the cultures within 2-3 h. The first cell to leave the initial explant was the first cell to leave the neural tube explant. The first cells leaving the initial explants were tightly organized neuroepithelial cells and the explants were firmly attached to the fibronectin layer.
• Cell Research:
  To determine the effect of FA supplementation on BRCA1 and BRCA2 mRNA expression, all cell lines were treated with 0, 25, 50, 75, or 100 nmol/L FA for 72 hours before harvesting in TRI Reagent according to the manufacturer’s instructions. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, EndogenousMetabolite, Folic acid, folic deficiency, Folacin, Folate, Inhibitor, inhibit, neural tube defects, nutrient, ntidepressant-like, DNA Synthesis, DNA/RNA Synthesis, DNASynthesis, Vitamin M, Vitamin B9, Vitamin B-9, Vitamin B 9, VB9, Pteroylglutamic acid, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

135444779