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| Catalog Number | orb782780 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Folic Acid(FA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Folic Acid(FA). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 156.25-10000 pg/mL |
| Assay Time | 2.5h |
| Concentration | 10000 pg/mL |
| Sensitivity | 15 pg/mL |
| Alternative names | VB9 ELISA Kit, Vitamin B9 ELISA Kit, Folacin ELISA Read more... |
| Note | For research use only |
| Application notes | standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human FA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
Human Folic acid ELISA Kit [orb339862]
Human
12.5 pg/mL-200 pg/mL
6.25 pg/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
