Fluoxetine

SKU: orb1310603

Description

Fluoxetine is a selective serotonin reuptake inhibitor (SSRI) with established antidepressant activity. It is widely used in neuroscience research to study serotonin signaling in both in vitro binding assays and in vivo models of depression and anxiety.

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 54910-89-3
• MW: 309.33
• Purity: 99.07%
• Formula: C₁₇H₁₈F₃NO
• SMILES: CNCCC(Oc1ccc(cc1)C(F)(F)F)c1ccccc1
• Target: Serotonin Transporter,Autophagy,MRP,5-HT Receptor
• Solubility: DMSO:237.5 mg/mL (767.79 mM);5% DMSO+95% Saline:0.3 mg/mL (0.97 mM)

Bioactivity

• Target IC50:
  MDA-MB-436 cells:0.5 μM|Ventricular myocyte cells:2.82 μM|JAR cells:32.4 nM|HEK293 cells:1020 nM|CHO cells:13.1 μM|HeLa cells:10 μM|MDA-MB-231 cells:0.5 μM
• In Vivo:
  METHODS: To assay antidepressant activity in vivo, Fluoxetine (2.5-10 mg/kg) was administered intraperitoneally to MRL/MpJ mice twice daily for twenty-six days. RESULTS: Chronic treatment with 5 and 10 mg/kg Fluoxetine significantly increased cell proliferation and BDNF levels in the hippocampus. Only chronic treatment with the highest Fluoxetine increased BDNF levels in the frontal cortex. METHODS: To test antidepressant activity in vivo, Fluoxetine (18 mg/kg) was administered orally once daily for three weeks to a model of corticosterone-induced anxiety/depression-like behavior in C57BL/6Ntac mice. RESULTS: Chronic Fluoxetine treatment reversed the anxiety phenotype. Regarding total distance traveled, chronic corticosterone treatment showed a nonsignificant trend that was eliminated by chronic Fluoxetine treatment.
• In Vitro:
  METHODS: Mouse cortical near-pure neuronal cell cultures were treated with Fluoxetine (3-30 µM) for 24 h. Cell viability was measured by MTT assay. RESULTS: Fluoxetine induced neuronal death in a concentration-dependent manner. METHODS: Human gastric cancer cells AGS were treated with Fluoxetine (10-20 µM) for 24 h. Apoptosis was detected by Flow cytometry. RESULTS: The number of early apoptotic cells in Fluoxetine-treated group was significantly increased by about 4-fold and 10-fold, respectively.

Storage & Handling

• Storage: keep away from direct sunlight | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5HTReceptor, 5-HTT, 5HT Receptor, Autophagy, LY110140, LY-110140, LY 110140, Inhibitor, inhibit, MRP1, Fluoxetine, SLC6A4, Serotonin Transporter, SerotoninTransporter, Sert (Sodium-dependent), SERT

Compound Identifiers

PubChem CID

3386