FluoroSpot Plus: Human IFN-gamma/IL-10 Discontinued

SKU: orb1532847

Description

The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-10 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting either one or both analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. Pre-coated plates enable a more rapid assay with minimized variability.

Key Properties

• Reactivity: Human

Storage & Handling

• Storage: 12 months
• Form/Appearance: pre-coated plates
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Yixin Qu 1 2 3, Wuyou Gao 1 2 3, Danping Huang 1 2 3, Xingyi Li 1 2 3, Rongxin Chen 1 2 3, Zhen Mao 1 2 3, Xianchai Lin Experimental Study of Ultra-Pulsed CO2 Fractional Laser Combined With Recombinant Human Epidermal Growth Factor Gel in the Treatment of Eyelid Keloid Ophthalmic Plast Reconstr Surg, (2024)

PubMed: 39197180

Applications

ELISA