FluoroSpot Plus: Human IFN-gamma/IL-10/IL-17A Discontinued

SKU: orb1532842

Description

The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-γ/IL-10/IL-17A FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes and includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. Pre-coated plates enable a more rapid assay with minimized variability.

Key Properties

• Reactivity: Human

Storage & Handling

• Storage: 12 months
• Form/Appearance: pre-coated plates
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Ling Lin 1, Quan Luo 1, Xinjing Gao 2, Qian Li 1, Wei Li 1, Xin Zhou 1, Weiyu Liu 1, Xuelian Zhong 1, Yunqing Yang 1, Xibao Zhang Correlation and Clinical Significance of HBD-2 and CXCL-1/2 Levels at Skin Lesions with Psoriasis Vulgaris Severity Immunol Invest, (2024)

PubMed: 39301953