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Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

Fluorescein Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

Catalog Number: orb1675118

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1675118
CategoryAntibodies
DescriptionMinimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins
Species/HostGoat
Tested applicationsFACS
ReactivityRat
Antibody TypeSecondary Antibody
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(ab)2 F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab)2 F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
ConjugationUnconjugated
StorageThis product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Buffer/Preservatives0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
NoteFor research use only
Application notesNot specified. Optimal titers for applications should be determined by the researcher.
Expiration Date12 months from date of receipt.