FLT4 Antibody

• Catalog Number: orb1254427
• Category: Antibodies
• Description: FLT4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1174-1363 of human FLT4 (NP_891555.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 153kDa
• Target: FLT4
• UniProt ID: P35916
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLT4, FLT41, Fms-related tyrosine kinase 4, Fms-li
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse liver, using FLT4 antibody (orb1254427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon using FLT4 antibody (orb1254427) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using FLT4 antibody (orb1254427) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using FLT4 antibody (orb1254427) at dilution of 1:100. Blue: DAPI for nuclear staining.