Flt-1 (phospho Tyr1333) rabbit pAb

SKU: orb764360

Description

Rabbit polyclonal antibody to Flt-1 (phospho-Tyr1333)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human VEGFR1 around the phosphorylation site of Tyr1333. AA range:1289-1338
• Molecular Weight: 151
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FLT1 antibody, anti-FLT antibody, anti-FRT antibody, anti-VEGFR1 antibody, anti-Vascular endothelial growth factor receptor 1 antibody, anti-VEGFR-1 antibody, anti-Fms-like tyrosine kinase 1 antibody, anti-FLT-1 antibody, anti-Tyrosine-protein kinase FRT antibody, anti-Tyrosine-protein kinase receptor FLT antibody, anti-FLT antibody, anti-Vascular perm antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.

Western Blot analysis of various cells using Phospho-Flt-1 (Y1333) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VEGFR1 (Phospho-Tyr1333) Antibody.

Immunohistochemistry analysis of paraffin-embedded human brain, using VEGFR1 (Phospho-Tyr1333) Antibody. The picture on the right is blocked with the phospho peptide.