FLNB Antibody

• Catalog Number: orb1258159
• Category: Antibodies
• Description: FLNB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 298kDa
• Target: FLNB
• UniProt ID: O75369
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FLNB, filamin B, beta (actin binding protein 278),
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FLNB antibody (orb1258159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HUVEC cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.