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FLNB Antibody
SKU: orb1258159
Featured
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1). |
| Target | FLNB |
| Molecular Weight | Observed: 298kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−FLNB, filamin B, beta (actin binding protein 278), ABP-278, AOI, DKFZp686A1668, DKFZp686O033, FH1, FLN1L, LRS1, SCT, TABP, TAP, beta filamin, filamin 1 (actin-binding protein-280)-like, beta (actin-binding protein-278), FLN3
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Western blot analysis of extracts of various cell lines, using FLNB antibody (orb1258159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HUVEC cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
FLNB
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
FLNB Antibody (orb1258159)
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