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FLNB Antibody

Catalog Number: orb1258159

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1258159
CategoryAntibodies
DescriptionFLNB Antibody
TargetFLNB
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid
Concentrationbatch dependent
Buffer/PreservativesLiquid
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
UniProt IDO75369
MWObserved: 298kDa
Tested applicationsIF, IHC, WB
Application notesWB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:20 - 1:100
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesFLNB, filamin B, beta (actin binding protein 278),
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NoteFor research use only
FLNB Antibody

Western blot analysis of extracts of various cell lines, using FLNB antibody (orb1258159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

FLNB Antibody

Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

FLNB Antibody

Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

FLNB Antibody

Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens).

FLNB Antibody

Immunofluorescence analysis of HeLa cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

FLNB Antibody

Immunofluorescence analysis of HUVEC cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

FLNB Antibody

Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

FLNB Antibody

Immunofluorescence analysis of PC-12 cells using FLNB antibody (orb1258159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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