Flg (phospho Tyr654) rabbit pAb

• Catalog Number: orb768119
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Flg (phospho-Tyr654)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FGFR1 around the phosphorylation site of Tyr654. AA range:626-675
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 120
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-FGFR1 antibody, anti-BFGFR antibody, anti-CEK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of Hela cells using Phospho-Flg (Y654) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FGFR1 (Phospho-Tyr654) Antibody.

Immunofluorescence analysis of COS7 cells, using FGFR1 (Phospho-Tyr654) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 15', using FGFR1 (Phospho-Tyr654) Antibody. The lane on the right is blocked with the phospho peptide.