FKBP3 Antibody

SKU: orb1256732

Description

FKBP3 Antibody

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).
• Target: FKBP3
• Molecular Weight: Observed: 30kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

FKBP3, 25 kDa FK506-binding protein, 25 kDa FKBP, FKBP-3, Immunophilin FKBP25, FK506-binding protein 3, FK506-binding protein 3 (25kD), PPIase FKBP3, Rapamycin binding protein, Rotamase, PPIase, FK506 binding protein 3, 25kDa, FKBP-25, FKBP25

Western blot analysis of extracts of various cell lines, using FKBP3 antibody (orb1256732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using FKBP3 antibody (orb1256732). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (orb1256732). Western blot was performed from the immunoprecipitate using FKBP3 antibody (orb1256732) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

FKBP3

UniProt

Q00688