FKBP3 Antibody

• Catalog Number: orb1256732
• Category: Antibodies
• Description: FKBP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human FKBP3 (NP_002004.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa
• Target: FKBP3
• UniProt ID: Q00688
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: FKBP3, 25 kDa FK506-binding protein, 25 kDa FKBP,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FKBP3 antibody (orb1256732) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using FKBP3 antibody (orb1256732). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells, using 3 ug FKBP3 antibody (orb1256732). Western blot was performed from the immunoprecipitate using FKBP3 antibody (orb1256732) at a dilution of 1:1000.