Filamin 1 rabbit pAb Antibody

SKU: orb765220

Description

Rabbit polyclonal antibody to Filamin 1

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Filamin A. AA range:2121-2170
• Molecular Weight: 280
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FLNA antibody, anti-FLN antibody, anti-FLN1 antibody, anti-Filamin-A antibody, anti-FLN-A antibody, anti-Actin-binding protein 280 antibody, anti-ABP-280 antibody, anti-Alpha-filamin antibody, anti-Endothelial actin-binding protein antibody, anti-Filamin-1 antibody, anti-Non-muscle filamin antibody

Western Blot analysis of various cells using Filamin 1 Polyclonal Antibody diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Filamin A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 5', using Filamin A Antibody. The lane on the right is blocked with the synthesized peptide.