FLG Antibody (Biotin)

SKU: orb751156

Description

Mouse monoclonal antibody against FLG conjugated to Biotin

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: U-251-MGcells.Human skin

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: FLG/1563
• Immunogen: Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)
• Target: FLG
• Source/Expression System: Bioreactor
• Molecular Weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ATOD2 antibody, anti-Epidermal Filaggrin antibody, anti-Filaggrin antibody, anti-Filaggrin precursor antibody, anti-Fillagrin antibody, anti-FLG antibody, anti-Profilaggrin antibody

Quick Database Links

Gene Symbol

FLG

UniProt

P20930

NCBI Gene ID

2312