Fibronectin, bovine Antibody

SKU: orb27334

Description

Rabbit polyclonal antibody to Fibronectin

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, RIA
• Dilution Range: (purified, lyophilized) IFA 1:80, IHC(P) 1:500 (Envision), ELISA 1:200 (OD>500)
• Application Notes:
  Positive Control: Bovine skin or liver. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Immunogen: Purified fibronectin from bovine plasma
• Purification: Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Concentration: app. 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunohistochemical staining of bovine skin using Fibronectin antibody

Quick Database Links

UniProt

P07589