Fibrinogen Alpha Chain Antibody

SKU: orb2643254

Description

The plasma glycoprotein Fibrinogen is synthesized in the liver and comprises three structurally different subunits. Fibrinogen is important in platelet aggregation, the final step of the coagulation cascade (i.e. the formation of Fibrin) and determination of plasma viscosity and erythrocyte aggregation. It is both constitutively expressed and inducible during an acute phase reaction. Hemostasis following tissue injury deploys essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Following vascular injury, Fibrinogen is cleaved by Thrombin to form Fibrin, which is the most abundant component of blood clots. The cleavage products of Fibrinogen regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types.

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/10^6 cells in 0.1ml,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Fibrinogen Alpha Chain antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: UC45
• Immunogen: Human acute monoblastic leukemia cells were used as the immunogen for the Fibrinogen Alpha Chain antibody.
• Purification: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P02671