Human Fibrinogen Alpha (FGa) ELISA Kit

SKU: orb775659

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibrinogen Alpha(FGa).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FGa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FGa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FGa in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 6.7 ng/mL
• Concentration: 1000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Fib2 ELISA Kit, FG-A ELISA Kit, Fibrinopeptide A ELISA Kit, Fibrinogen Alpha chain ELISA Kit, FGB ELISA Kit

Quick Database Links

UniProt

P02671