FIBG Antibody (C-term)

SKU: orb1929965

Description

FIBG Antibody (C-term)

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 51512 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Confocal immunofluorescent analysis of FIBG Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

FIBG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FIBG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of FIBG Antibody (C-term) in NCI-H460 cell line lysates (35 ug/lane). FIBG (arrow) was detected using the purified Pab.

Anti-FIBG Antibody (C-term) at 1:4000 dilution + A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P02679