FHL5 antibody [N1C2]

• Catalog Number: orb556403
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FHL5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 33
• Target: FHL5
• UniProt ID: Q5TD97
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: four and a half LIM domains 5 , 1700027G07Rik , AC
• Note: For research use only
• Application notes: Positive control: 293T , Raji
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using FHL5 antibody

Immunohistochemical staining of SW480 Xenograft using FHL5 antibody

Western blot analysis of 293T(Lane 1), Raji(Lane 2) using FHL5 antibody