FGL2 Antibody (C-term)

SKU: orb1938429

Description

FGL2 Antibody (C-term)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 50229 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-FGL2 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: KG-1 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-FGL2 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 54kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FGL2 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the FGL2 antibody detected the FGL2 protein (arrow).

FGL2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the FGL2 antibody detected the FGL2 protein (arrow).

Overlay histogram showing U-2OS cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

Q14314