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FGFR2 Antibody (N-term R22)
Description
Images & Validation
−| Tested Applications | FC, IF, WB |
|---|---|
| Dilution range | WB - 1:1000, IF - 1:10-50, FC - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 92025 Da |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35 ug/lane). FGFR2 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-FGFR2 Antibody (N-term R22) at 1:1000 dilution. Lane 1: human breast lysate. Lane 2: human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 92 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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UniProt Details
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Protocol Information
FGFR2 Antibody (N-term R22) (orb1929044)
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