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Item 1 of 4
FGFR2 Antibody (N-term R22)
Catalog Number: orb1929044
| Catalog Number | orb1929044 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB15689 |
| Tested applications | FC, IF, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 92025 Da |
| Target | This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human FGFR2. |
| UniProt ID | P21802 |
| NCBI | NP_001138388.1, NP_001138389.1, NP_075259.4, NP_001138391.1, NP_001138386.1, NP_000132.3, NP_001138385.1, NP_001138387.1, NP_001138390.1, NP_075418.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Fibroblast growth factor receptor 2, FGFR-2, K-sam Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35 ug/lane). FGFR2 (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-FGFR2 Antibody (N-term R22) at 1:1000 dilution. Lane 1: human breast lysate. Lane 2: human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 92 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
