FGFR1 Antibody (N-term)

SKU: orb1929052

Description

Research Area

Cancer Biology, Cardiovascular Research, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	FC - 1:10-50, WB - 1:1000, IHC-P - 1:100-500, IF - 1:50-100
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	91868 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

The anti-FGFR1 Pab is used in Western blot to detect FGFR1 in NIH-3T3 cell lysate.

Flow cytometric analysis of MCF-7 cells using FGFR1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i, iv, vii), A1B3 is visualized as green fluorescence (ii, v, viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii, vi, ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25.