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Item 1 of 8
Item 1 of 8
FGF21 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb2563504
| Catalog Number | orb2563504 |
|---|---|
| Category | Antibodies |
| Description | FGF21 Recombinant Rabbit Monoclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Recombinant |
| Clone Number | 7C4 |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human FGF21 (1-100/208aa) |
| Antibody Type | Recombinant Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 20 kDa |
| Target | FGF21 |
| UniProt ID | Q9NSA1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Fibroblast growth factor 21; FGF 21; FGF-21; FGFL; Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
25 ug total protein per lane of various lysates probed with FGF21 monoclonal antibody, unconjugated (orb2563504) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
