FGF21 Recombinant Rabbit Monoclonal Antibody

SKU: orb2563504

Description

FGF21 Recombinant Rabbit Monoclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 7C4
• Immunogen: A synthesized peptide derived from human FGF21 (5-61aa)
• Target: FGF21
• Molecular Weight: 20 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Fibroblast growth factor 21; FGF 21; FGF-21; FGFL; FGF21_HUMAN.

25 ug total protein per lane of various lysates probed with FGF21 monoclonal antibody, unconjugated (orb2563504) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with FGF21 Monoclonal Antibody, Unconjugated (orb2563504) at 1:250 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

FGF21

UniProt

Q9NSA1