FEZ1 Antibody

• Catalog Number: orb1247516
• Category: Antibodies
• Description: FEZ1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QNKQKEQRELMKK
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FEZ1
• UniProt ID: Q99689
• NCBI: NP_005094.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FEZ1, zygin 1, fasciculation and elongation protei
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 45.1kDa according to NP_005094.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Colon and Brain (Cortex). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247516 (0.03 ug/ml) staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247516 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247516 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.