FEN-1 rabbit pAb

SKU: orb765211

Description

Rabbit polyclonal antibody to FEN-1

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human FEN1. AA range:86-135
• Molecular Weight: 42
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-FEN1 antibody, anti-RAD2 antibody, anti-Flap endonuclease 1 antibody, anti-FEN-1 antibody, anti-DNase IV antibody, anti-Flap structure-specific endonuclease 1 antibody, anti-Maturation factor 1 antibody, anti-MF1 antibody, anti-hFEN-1 antibody

Western Blot analysis of various cells using FEN-1 Polyclonal Antibody diluted at 1:500.

Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).