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Catalog Number | orb1937367 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31101 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 32903 Da |
Target | This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 amino acids from the C-terminal region of human FCN3. |
UniProt ID | O75636 |
NCBI | NP_775628.1, NP_003656.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Ficolin-3, Collagen/fibrinogen domain-containing l Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-FCN3 Antibody (C-term) at 1:500 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
FCN3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
FCN3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of FCN3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
FCN3 Antibody (C-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the FCN3 antibody detected the FCN3 protein (arrow).