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FCGR3B Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human FCGR3B (NP_000561.3). |
| Target | FCGR3B |
| Molecular Weight | Observed: 50kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human Fc Fragment of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit [orb776662]
Human
0.32-20 ng/mL
0.131 ng/mL
96 T, 48 T

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Western blot analysis of extracts of various cell lines, using FCGR3B antibody (orb1260153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using FCGR3B antibody (orb1260153) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Protocol Information
FCGR3B Antibody (orb1260153)
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