FCGR3B Antibody

• Catalog Number: orb1260153
• Category: Antibodies
• Description: FCGR3B Antibody
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human FCGR3B (NP_000561.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 50kDa
• Target: FCGR3B
• UniProt ID: O75015
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Low affinity immunoglobulin gamma Fc region recept
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FCGR3B antibody (orb1260153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using FCGR3B antibody (orb1260153) at dilution of 1:100. Blue: DAPI for nuclear staining.