FBXL5 Antibody (N-term)

SKU: orb1927933

Description

FBXL5 Antibody (N-term)

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: IHC-P - 1:100, WB - 1:1000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 78555 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

FBL4, FBL5, FLR1

Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35 ug/lane). FBXL5 (arrow) was detected using the purified Pab.

FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-FBXL5 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Quick Database Links

UniProt

Q9UKA1