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FBXL3 Antibody

SKU: orb1537877

Description

FBXL3 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution rangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (1 - 3 µg/ml)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: LS-B2770 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2770 was determined to be 5 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence KRGGRDSDRNSSEE-C, from the N-Terminus of protein sequence according to NP_036290.1.
TargetFBXL3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

FBXL3, F-box/LRR-repeat protein 3, F-box/LRR-repeat protein 3A, FBL3, FBXL3A, F-box protein Fbl3a, FBL3A

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

FBXL3 Antibody

Antibody (1 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FBXL3 Antibody (orb1537877)

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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