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    FBN1 Antibody

    Catalog Number: orb1249415

    DispatchUsually dispatched within 5-10 working days
    $ 813.00
    Catalog Numberorb1249415
    CategoryAntibodies
    DescriptionFBN1 Antibody
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, FC, IF
    Predicted ReactivityBovine, Canine, Mouse, Rat
    ReactivityHuman
    ImmunogenThe immunogen for this antibody is: C-DASNIEDQSETEAN
    Concentration500 ug/mL
    Dilution rangePeptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Heart, Kidney, Skeletal Muscle and Skin lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and Jurkat cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
    Form/AppearanceLiquid
    ConjugationUnconjugated
    TargetFBN1
    UniProt IDP35555
    NCBINP_000129.3
    StorageAliquot and store at -20°C. Minimize freezing and thawing.
    Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
    Alternative namesFBN1, fibrillin 1, FBN, MASS, MFS1, OCTD, SGS, WMS
    Read more...
    NoteFor research use only
    Application notesPeptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Heart, Kidney, Skeletal Muscle and Skin lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and Jurkat cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
    Expiration Date12 months from date of receipt.
    FBN1 Antibody

    orb1249415 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

    FBN1 Antibody

    orb1249415 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

    FBN1 Antibody

    orb1249415 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).

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