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Catalog Number | orb1249415 |
---|---|
Category | Antibodies |
Description | FBN1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-DASNIEDQSETEAN |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Heart, Kidney, Skeletal Muscle and Skin lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and Jurkat cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FBN1 |
UniProt ID | P35555 |
NCBI | NP_000129.3 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | FBN1, fibrillin 1, FBN, MASS, MFS1, OCTD, SGS, WMS Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Heart, Kidney, Skeletal Muscle and Skin lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and Jurkat cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249415 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
orb1249415 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.
orb1249415 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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