FBN1 Antibody

• Catalog Number: orb1249415
• Category: Antibodies
• Description: FBN1 Antibody
• Target: FBN1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DASNIEDQSETEAN
• UniProt ID: P35555
• Tested applications: ELISA, FC, IF
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Brain (Cerebellum), Heart, Kidney, Skeletal Muscle and Skin lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and Jurkat cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: FBN1, fibrillin 1, FBN, MASS, MFS1, OCTD, SGS, WMS
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_000129.3

orb1249415 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1249415 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1249415 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).