Fatostatin hydrobromide

SKU: orb1300425

Description

Fatostatin hydrobromide

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 298197-04-3
• MW: 375.33
• Purity: 98.62%
• Formula: C₁₈H₁₈N₂S·HBr
• SMILES: Br.CCCc1cc(ccn1)-c1nc(cs1)-c1ccc(C)cc1
• Target: Fatty Acid Synthase,NPC1L1
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:58 mg/mL (154.53 mM);DMSO:3.76 mg/mL (10.02 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.66 mM)

Bioactivity

• In Vivo:
  Fatostatin significantly inhibits subcutaneous C4-2B tumor growth and markedly decreases serum PSA level compared to the control group. Fatostatin blocks increases in body weight, blood glucose, and hepatic fat accumulation in obese ob/ob mice, even under uncontrolled food intake.
• In Vitro:
  Fatostatin inhibit the insulin-induced adipogenesis of 3T3-L1 cells an the serum-independent growth of human androgen-independent prostate cancer (DU145) cells. Fatostatin suppresses cell proliferation and anchorage-independent colony formation In both androgen-responsive LNCaP and androgen-insensitive C4-2B prostate cancer cells. Fatostatin block the activation of SREBPs in cells in tissue culture.Fatostatin also reduced in vitro invasion and migration In both cell lines. Further, fatostatin causes G2/M cell cycle arrest and induces apoptosis by increasing caspase-3/7 activity an the cleavages of caspase-3 and PARP.
• Cell Research:
  Cell lines: CHO-K1 cells. concentration : 20 μM. Incubation Time: 20 h. Method: On day 0,CHO-K1 cells are plated out onto a 96-well plate in medium A.On day 2,the cells are transiently cotransfected with pCMV-PLAP-BP2(513–1141),pCMV-SCAP and pAc-β-gal,using Lipofectamine reagent.After incubation for 5 hr,the cells are washed with PBS and then incubated in medium B,i the absence or presence of fatostatin (20 μM) or sterols (10 μg mL cholesterol and 1 μg mL 25 hydroxycholesterol).After 20 hr of incubation,an aliquot o the medium is assayed for secreted alkaline phosphatase activity.The cells in Each well are lysed and used for measurement of β-galactosidase activities.The alkaline phosphatase activity is normalized b the activity of β-galactosidase.
• Animal Research:
  Animal Models: Obese (ob/ob) mice (C57BL/6J background). Formulation: 10% DMSO in PBS. Dosages: 30 mg/kg. Administration: intraperitoneal injection

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

125B11, adipogenesis, insulin-induced, gene, Golgi, expression, Fatostatin HBr, Fatty Acid Synthase, Fatty Acid Synthase (FASN), FattyAcidSynthase, mice, ob/ob, SREBP-mediated, SREBPs

Compound Identifiers

PubChem CID

2850562