FANCG Antibody

• Catalog Number: orb1247530
• Category: Antibodies
• Description: FANCG Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: LEEFRTSLPKSCDL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa, and in nuclear lysates of cell line Jurkat (calculated MW of 68.6kDa according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Spleen. Recommended concentration: 3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FANCG
• UniProt ID: O15287
• NCBI: NP_004620.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FANCG, XRCC9, Fanconi anemia, complementation grou
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa, and in nuclear lysates of cell line Jurkat (calculated MW of 68.6kDa according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Spleen. Recommended concentration: 3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247530 (0.5 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing FANCG and probed with orb1247530 (mock transfection in first lane).

orb1247530 (1 ug/ml) staining of Jurkat nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247530 (3 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247530 (3 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.