FANCG Antibody

Catalog Number: orb1247530

Catalog Number	orb1247530
Category	Antibodies
Description	FANCG Antibody
Clonality	Polyclonal
Tested applications	ELISA, IHC, WB
Reactivity	Human
Immunogen	The immunogen for this antibody is: LEEFRTSLPKSCDL
Antibody Type	Primary Antibody
Concentration	500 ug/mL
Form/Appearance	Liquid
Conjugation	Unconjugated
Target	FANCG
UniProt ID	O15287
NCBI	NP_004620.1
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative names	FANCG, XRCC9, Fanconi anemia, complementation grou Read more...
Note	For research use only
Application notes	Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa, and in nuclear lysates of cell line Jurkat (calculated MW of 68.6kDa according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Spleen. Recommended concentration: 3ug/ml.
Expiration Date	12 months from date of receipt.

orb1247530 (0.5 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing FANCG and probed with orb1247530 (mock transfection in first lane).

orb1247530 (1 ug/ml) staining of Jurkat nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247530 (3 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247530 (3 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.