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Catalog Number | orb1246724 |
---|---|
Category | Antibodies |
Description | FAM62A Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-QKKRTLSPEFNER |
Antibody Type | Primary Antibody |
Concentration | 500 ug/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FAM62A |
UniProt ID | Q9BSJ8 |
NCBI | NP_056107.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | FAM62A, family with sequence similarity 62 (C2 dom Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 150kDa band observed in Human Adipose lysates (calculated MW of 123kDa according to NP_056107.1). In transfected HEK293 transiently expressing FAM62A a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246724 (0.5 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing FAM62A and probed with orb1246724 (mock transfection in first lane).
orb1246724 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IHC-P | |
Bovine, Equine, Guinea pig, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |