FAM168B Antibody (Center)

SKU: orb1926568

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: WB - 1:2000, IHC - 1:25, FC - 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD72078
• Immunogen: This FAM168B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 105-139 amino acids from the Central region of human FAM168B.
• Target: FAM168B
• Molecular Weight: 20324 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myelin-associated neurite-outgrowth inhibitor, Mani, p20, FAM168B, KIAA0280L, MANI

Anti-FAM168B Antibody (Center) at 1:2000 dilution + human brain lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining FAM168B in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing SH-SY5Y cells stained (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

FAM168B

UniProt

A1KXE4