FAM120A Antibody

SKU: orb1787873

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:2000, IHC-P: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Immunogen: This FAM120A antibody is generated from a mouse immunized with a recombinant protein of human FAM120A. Antigen Region: 280-600 aa.
• Target: FAM120A
• Molecular Weight: 121888 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator, Protein FAM120A, FAM120A, C9orf10, KIAA0183, OSSA

All lanes: Anti-FAM120A Antibody at1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 122 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining FAM120A in human brain sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

FAM120A

UniProt

Q9NZB2