PTK2 Antibody

SKU: orb1261286

Description

PTK2 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (NP_722560.1).
• Target: PTK2
• Molecular Weight: Observed: 120kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK

Western blot analysis of extracts of various cell lines, using PTK2 antibody (orb1261286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human colon using FAK antibody (orb1261286) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using FAK antibody (orb1261286) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of RAW264. 7 cells using FAK antibody (orb1261286) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug FAK antibody (orb1261286). Western blot was performed from the immunoprecipitate using FAK antibody (orb1261286) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

PTK2

UniProt

Q05397