FAK Antibody [E1E5]

• Catalog Number: orb1261299
• Category: Antibodies
• Description: FAK Antibody [E1E5]
• Target: PTK2
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant protein of human FAK
• UniProt ID: Q05397
• MW: Observed: 102kDa
• Tested applications: IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Antibody Type: Primary Antibody
• Clone Number: [E1E5]
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FA
• Note: For research use only

Western blot analysis of extracts of various cell lines, using FAK antibody (orb1261299). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse spleen using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human spleen using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using FAK antibody (orb1261299) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of SH-SY-5Y cells using FAK antibody (orb1261299).

Immunofluorescence analysis of PANC-1 cells using FAK antibody (orb1261299).