FADS2 Antibody

• Catalog Number: orb1925479
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This FADS2 antibody is generated from a rabbit immunized with a recombinant protein of human FADS2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: O95864
• MW: 52259 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:2000
• Antibody Type: Primary Antibody
• Clone Number: RB56816
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fatty acid desaturase 2, 1.14.19.-, Delta(6) fatty
• Note: For research use only

Anti-FADS2 Antibody at 1:2000 dilution + Human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeling FADS2 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1186255) at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).