Factor XIII alpha antibody

SKU: orb2276332

Description

Factor XIII alpha antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A fraction of monocytic cells in the mucosa should show a strong cytoplasmic factor XIIIa immunostaining. Negative Control: Colon: Factor XIIIa immunostaining should be absent in epithelial, endothelial and smooth muscle cells. Cellular Localization: Cytoplasmic and Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-813R
• Immunogen: Recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; F13A; F13a1; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase. Plasma

Quick Database Links

UniProt

P00488