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F(ab')2 Sheep IgG Fab'2 Antibody Peroxidase Conjugated
Catalog Number: orb346805
| Catalog Number | orb346805 |
|---|---|
| Category | Antibodies |
| Description | F(ab')2 Sheep IgG Fab'2 antibody (Peroxidase) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG F(ab')2 |
| Conjugation | HRP |
| Reactivity | Sheep |
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Purity | This product is a F(ab’)2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin. |
| Immunogen | Sheep IgG F(ab')2 fragment |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000 |
| Application notes | F(ab')2 Anti-Sheep IgG Fab'2 Peroxidase secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit F(ab')2 anti-sheep IgG F(ab')2 Peroxidase C Read more... |
| Note | For research use only |
