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F(ab')2 Rat IgG (H&L) AntibodyPre-Adsorbed

F(ab')2 Rat IgG (H&L) AntibodyPre-Adsorbed

Catalog Number: orb348371

DispatchUsually dispatched within 5-10 working days
$ 370.00
Catalog Numberorb348371
CategoryAntibodies
DescriptionF(ab')2 Rat IgG (H&L) antibody
Species/HostDonkey
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityRat
IsotypeIgG F(ab')2
ImmunogenRat IgG whole molecule
Antibody TypeSecondary Antibody
Concentration1.36 mg/mL
Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/AppearanceLiquid (sterile filtered)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rat IgG, and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Sheep Serum Proteins.
ConjugationUnconjugated
StorageStore vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesDonkey F(ab')2 Anti-Rat IgG Pre-Adsorbed Antibody,
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NoteFor research use only
Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Expiration Date12 months from date of receipt.