F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin conjugated

SKU: orb348337

Description

F(ab')2 Rat IgG (H&L) antibody (RPE)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Reactivity: Rat
• Application Notes:
  F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Antibody has been tested by western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Rat IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.5 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Rat IgG Phycoerythrin Conjugated Antibody, Goat Fab2 Anti-Rat IgG Antibody PE Conjugation