F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

• Catalog Number: orb348284
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody (FITC)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Mouse
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey F(ab')2 Anti-Mouse IgG Antibody fluorescein
• Note: For research use only
• Application notes: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

HSPB8 silencing and its effect on expression of ERα and ERβin MCF-7 cell line. Representative pictures of immunofluorescence staining of HSPB8 (red, anti-rabbit p/n orb347751, 1:500) and α-tubulin (green, anti-mouse p/n orb348284, 1:500) in MCF-7 cells transfected for 1 and 2 days. DAPI (blue) was used to stain DNA C.