Cart summary

You have no items in your shopping cart.

F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Catalog Number: orb348284

DispatchUsually dispatched within 5-10 working days
$ 580.00
Catalog Numberorb348284
CategoryAntibodies
DescriptionF(ab')2 Mouse IgG (H&L) antibody (FITC)
ClonalityPolyclonal
Species/HostDonkey
IsotypeIgG F(ab')2
ConjugationFITC
ReactivityMouse
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
ImmunogenMouse IgG whole molecule
Tested applicationsFC, FLISA, IF
Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesDonkey F(ab')2 Anti-Mouse IgG Antibody fluorescein
Read more...
NoteFor research use only
F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

HSPB8 silencing and its effect on expression of ERα and ERβin MCF-7 cell line. Representative pictures of immunofluorescence staining of HSPB8 (red, anti-rabbit p/n orb347751, 1:500) and α-tubulin (green, anti-mouse p/n orb348284, 1:500) in MCF-7 cells transfected for 1 and 2 days. DAPI (blue) was used to stain DNA C.