F(ab')2 Mouse IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

• Catalog Number: orb348236
• Category: Antibodies
• Description: F(ab')2 Mouse IgG (H&L) antibody (Biotin)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG F(ab')2
• Conjugation: Biotin
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins.
• Immunogen: Mouse IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:15,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat F(ab')2 Anti-Mouse IgG Antibody Biotin Conjug
• Note: For research use only