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Catalog Number | orb348253 |
---|---|
Category | Antibodies |
Description | F(ab')2 Mouse IgG F(ab')2 antibody (Peroxidase) |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Mouse |
Isotype | IgG F(ab')2 |
Immunogen | F(ab')2 Anti-Mouse IgG F(ab')2 fragment was produced by repeated immunization with Mouse IgG F(ab')2 fragment in goat. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:130,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 to 1:20,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | Goat F(ab')2 Anti-Mouse IgG F(ab')2 Antibody perox Read more... |
Note | For research use only |
Application notes | F(ab')2 Anti-Mouse IgG F(ab')2 fragment Peroxidase conjugated has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Expiration Date | 12 months from date of receipt. |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin. | |
Mouse |
ELISA, IHC, WB | |
Mouse | |
Goat | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
HRP |
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